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. 2017 Nov 16;8:2257. doi: 10.3389/fmicb.2017.02257

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Copyright © 2017 Lin, Paff, Molineux and Bull.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and purification of recombinant K1, K5, and K30 depolymerases. (A) SDS-PAGE showing induced expression and purification of K1E, K1F, and K1H depolymerases. 50 μg of uninduced or induced whole cell lysate obtained after sonication, or 2 μg of enzyme purified was fractionated by SDS-PAGE followed by Coomassie Blue staining. U, uninduced whole cell lysate; I, induced whole cell lysate; P, purified enzyme. (B) SDS-PAGE of K5 and putative K30 depolymerases after a similar induction and protein purification.