Skip to main content
. 2017 Nov 21;10:389. doi: 10.3389/fnmol.2017.00389

FIGURE 6.

FIGURE 6

Immunofluorescence and/or western blot analyses of NGF and TrkA in sections of the ipsilateral lumbar DRGs (L4–L6) from CCI-rats administered a single bolus dose of EMA300 (10 mg kg-1) or vehicle relative to the corresponding sections from sham-rats. (A,B) During IHC, the expression level of total NGF was not significantly altered in CCI-rats among all treatment groups (F(2,31) = 3.69; P > 0.05) relative to sham-rats. However, the expression of TrkA was elevated by ∼3-fold (F(2,28) = 14.37; P ≤ 0.05) in vehicle-treated CCI rats that was not changed by EMA300. (C,D) The western blot analyses of NGF revealed that in the ipsilateral lumbar DRGs of CCI-rats, expression of the pro- and mature-NGF isoforms were significantly reduced (F(2,9) = 11.95; P ≤ 0.05) and that expression of mature-NGF was rescued at the time of peak effect of a single i.p. bolus dose of EMA300 (10 mg kg-1) to match the corresponding mature NGF expression level in sham-rats. (One-way ANOVA followed by Tukey’s multiple comparison test). Scale bars represent 50 μm. CCI, Chronic constriction injury, kDa, kiloDaltons; Veh, vehicle.