Fig. 7.

Overexpression of TaMYB80 confers heat tolerance to transgenic Arabidopsis plants. a Schematic diagram of the TaMYB80 overexpression construct driven by the 35S promoter. b Analysis of TaMYB80 transcript levels in WT Arabidopsis and transgenic lines (L1, L2, and L3). Total RNA was isolated from 1-week-old plants. Actin2 was used as the control gene. c Germination rates (%) of TaMYB80-overexpression plants after treatment with heat stress. Seeds of WT and transgenic lines were sterilized and subjected to 50 °C treatment for 1 h and then germinated on 1% agar plates. The plates were placed at ambient conditions (22 °C) for 7 d before photographs were taken. At least 50 seeds were assessed per replicate for five independent replicates. d Survival rates (%) of WT and transgenic seedlings after treatment with heat stress. Five-day-old seedlings were heat-treated at 45 °C for 1 h before returning to 22 °C to grow for two days, and then photographs were taken and the survival rates were calculated. More than 40 plants of each line were used. Bars represent standard deviations