Table 2.
Full set of fruit proteins differentially abundant in (A) cv. Aragon, (B) cv. Gladis
| aspot n° | bProtein name | cGene name | dOrganism | eScore | fCov. % | gExp. Mr. (kDa)/pI | hTh. Mr. (kDa)/pI | iBLAST protein | l gene name | midentity % | nTh. Mr. (kDa) | oCov. % |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A) | ||||||||||||
| 202 | UPF0725 protein At1g27860 | At1g27860 | A. thaliana | 31 | 22 | 31/4,5 | 33,4/5,41 | uncharacterized protein LOC101252956 (predicted) | Solyc03g005820.2 | 29 | 25,5 | 34 |
| 1204 | 14-3-3 protein 2 | Solyc12g057110.2 | S. lycopersicum | 32 | 22 | 30/4,5 | 29,0/4,72 | |||||
| 2103 | calmodulin-like protein 3 | CML3 | O. sativa | 24 | 44 | 23/5,0 | 20,3/4,62 | calmodulin | Solyc03g098050.2 | 65 | 16,8 | 84 |
| 3405 | actin-51 (Fragment) | Solyc11g005330.1 | S. Lycopersicum | 110 | 41 | 40/5,5 | 37,3/5,28 | |||||
| 3602 | probable lipid-A-disaccharide synthase, mitochondrial | LPXB | A. thaliana | 27 | 7 | 55/5,5 | 51,9/9,0 | probable lipid-A-disaccharide synthase, mitochondrial (predicted) | Solyc07g049740.2 | 60 | 51,8 | 94 |
| 4302 | annexin p34 | Solyc04g073990.2 | S. Lycopersicum | 100 | 88 | 35/5,5 | 35,7/6,41 | |||||
| 4803 | ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic | Solyc12g042060.1 | S. Lycopersicum | 28 | 28 | >97/5,5 | 102,5/5,8 | |||||
| 5004 | pathogenesis-related protein STH-2-like | Solyc09g090980.2 | S. Lycopersicum | 78 | 98 | 18/5,5 | 17,2/5,5 | |||||
| 5305 | cyclin-D6-1 | CYCD6-1 | T. aestivum | 42 | 12 | 35/5,8 | 34,6/5,5 | putative cyclin-D6-1 | Solyc07g054950.1 | 33 | 35,4 | 80 |
| 5602 | seed biotin-containing protein SBP65 | SBP65 | P. sativum | 75 | 42 | 60/5,8 | 59,6/5,9 | seed biotin-containing protein SBP65-like isoform X1 | Solyc10g008040.2 | 34 | 66,1 | 62 |
| 5702 | flavonol sulfotransferase-like | N/A | F. bidentis | 68 | 49 | 66/6,0 | 36,2/5,8 | cytosolic sulfotransferase 12-like (predicted) | Solyc05g011870.1 | 45 | 38,6 | 98 |
| 5706 | nucleolin 2 | NUCL2 | A. thaliana | 41 | 22 | 70/6,2 | 69,0/5,0 | nucleolin 1 isoform X1 (predicted) | Solyc02g021220.1 | 47 | 65,7 | 53 |
| 6101 | eukaryotic translation initiation factor 5A-2 | Solyc07g005560.2.1 | S. Lycopersicum | 89 | 76 | 20/6,5 | 17,7/5,8 | |||||
| 6102 | small heat shock protein, chloroplastic | Solyc03g082420.2 | S. Lycopersicum | 67 | 56 | 25/6,3 | 26,3/7,8 | |||||
| 6103 | PHD finger protein ALFIN-LIKE 3 | OsI_14081 | O. sativa | 50 | 44 | 25/6,5 | 28,3/5,7 | PHD finger protein ALFIN-LIKE 2-like (predicted) | Solyc10g076690.1 | 70 | 27 | 93 |
| 6303 | ADP, ATP carrier protein 2, mitochondrial | ANT-G2 | T. aestivum | 47 | 48 | 35/6,2 | 35,9/9,0 | ADP/ATP translocator | Solyc11g062130.1 | 88 | 41,9 | 98 |
| 7103 | protein LURP-one-related 1 | At1g33840 | A. thaliana | 41 | 37 | 23/6,6 | 25,7/8,6 | protein LURP-one-related 10-like (predicted) | Solyc10g085420.1 | 53 | 23,8 | 82 |
| 7503 | alcohol dehydrogenase 2 | ADH2 | S. Lycopersicum | 49 | 49 | 46/6,8 | 41,8/6,0 | |||||
| 7705 | F-box/WD-40 repeat-containing protein At5g21040 | At5g21040 | A. thaliana | 40 | 33 | 66/6,7 | 61,1/8,5 | F-box/WD-40 repeat-containing protein At5g21040-like (predicted) | Solyc06g073650.1 | 59 | 60,9 | 100 |
| 8105 | growth-regulating factor 10 | GRF10 | O. sativa | 43 | 16 | 22/6,8 | 22,5/9,0 | growth-regulating factor 9-like (predicted) | Solyc08g079800.2 | 59 | 24,6 | 51 |
| 8202 | VQ motif-containing protein 4 | VQ4 | A. thaliana | 39 | 32 | 28/6,7 | 26,96/9,8 | uncharacterized protein LOC101257285 (predicted) | Solyc02g078030.1 | 62 | 25,1 | 79 |
| 8701 | V-type proton ATPase catalytic subunit | N/A | B. vulgaris | 40 | 37 | 66/6,8 | 68,8/5,1 | vacuolar H + −ATPase A2 subunit isoform | Solyc06g063330.2 | 92 | 68,5 | 100 |
| B) | ||||||||||||
| 302 | E3 ubiquitin-protein ligase CHIP | CHIP | A. thaliana | 27 | 42 | 35/4,7 | 32,1/6,1 | E3 ubiquitin-protein ligase CHIP (predicted) | Solyc06g083150.2 | 69 | 31,6 | 97 |
| 502 | F-box protein At4g00755 | At4g00755 | A. thaliana | 24 | 22 | 44/4,4 | 43,1/4,9 | F-box protein At4g00755-like (predicted) | Solyc03g026170.2 | 54 | 41,1 | 81 |
| 1003 | Heavy metal-associated isoprenylated plant protein | HIPP26 | A. thaliana | 26 | 35 | 15/5,0 | 17,2/9,3 | heavy metal-associated isoprenylated plant protein 26-like (predicted) | Solyc01g111600.2 | 85 | 17,2 | 100 |
| 1305 | GDSL esterase/lipase EXL4 | EXL4 | A. thaliana | 18 | 17 | 40/5,1 | 38,3/9,0 | GDSL esterase/lipase EXL3-like (predicted) | Solyc04g082390.2 | 42 | 43,7 | 91 |
| 1401 | SKP1-like protein 21 | ASK21 | A. thaliana | 18 | 18 | 40/5,0 | 40,3/5,6 | SKP1-like protein 21 isoform X1 (predicted) | Solyc06g036070.2 | 65 | 40,1 | 96 |
| 1803 | heat shock 70 kDa protein | HSP70 | Z. mays | 36 | 26 | 70/5,2 | 70,8/5,22 | heat shock cognate 70 kDa protein 1 | Solyc10g086410.2 | 89 | 70,9 | 99 |
| 2802 | cell division cycle protein 48 homolog | CDC48 | G. max | 69 | 33 | 97/5,3 | 90,5/5,2 | cell division cycle protein 48 homolog (predicted) | Solyc06g074980.2 | 93 | 90,1 | 100 |
| 2805 | sucrose synthase 7 | SUS7 | O. sativa | 25 | 9 | 97/5,3 | 98,4/8,0 | sucrose synthase 7-like (predicted) | Solyc02g081300.2 | 69 | 96,2 | 98 |
| 3302 | dihydroflavonol-4-reductase | Solyc02g085020.2.1 | S. Lycopersicum | 24 | 25 | 35/5,4 | 42,7/6,0 | |||||
| 3401 | 1-aminocyclopropane-1-carboxylate oxidase homolog isoform 1 | Solyc09g089580.2.1 | S. Lycopersicum | 71 | 38 | 40/5,5 | 40,9/5,6 | |||||
| 3601 | ATP synthase subunit beta, chloroplastic | Solyc01g007320.2.1 | S. Lycopersicum | 48 | 43 | 50/5,4 | 53,5/5,3 | |||||
| 3801 | subtilisin-like protease SBT1.7 | SBT1.7 | A. thaliana | 26 | 15 | 97/5,5 | 80,1/5,9 | SBT1 protein precursor | Solyc04g078110.1 | 64 | 78,6 | 97 |
| 4702 | calcium-dependent protein kinase 18 | CPK18 | A. thaliana | 48 | 22 | 64/5,6 | 60,4/8,7 | calcium-dependent protein kinase | Solyc03g033540.2 | 80 | 63,7 | 89 |
| 5303 | ADP,ATP carrier protein 2, mitochondrial | ANT-G2 | T. aestivum | 47 | 48 | 35/6,2 | 35,9/9,0 | ADP/ATP translocator | Solyc11g062130.1 | 88 | 41,9 | 98 |
| 5601 | enolase | Solyc09g009020.2.1 | S. Lycopersicum | 147 | 64 | 48/5,8 | 48,0/5,7 | |||||
| 6502 | alcohol dehydrogenase 2 | Solyc06g059740.2.1 | S. Lycopersicum | 31 | 34 | 42/6,4 | 41,7/6,0 | |||||
| 6606 | CBL-interacting protein kinase 14 | CIPK14 | O. sativa | 37 | 26 | 49/6,4 | 50,5/9,0 | CBL-interacting protein kinase | Solyc05g052270.1 | 60 | 49,7 | 100 |
| 6607 | 26S protease regulatory subunit 7 homolog A | RPT1A | A. thaliana | 45 | 25 | 50/6,4 | 48,2/6,3 | 26S protease regulatory subunit 7 homolog A-like (predicted) | Solyc06g063140.2 | 98 | 47,7 | 99 |
| 6701 | phosphomethylpyrimidine synthase, chloroplastic | THIC | A. thaliana | 69 | 30 | 70/6,4 | 72,6/6,0 | phosphomethylpyrimidine synthase, chloroplastic (predicted) | Solyc06g006080.2 | 83 | 72,5 | 99 |
| 7203 | SufE-like protein 2, chloroplastic | SUFE2 | A. thaliana | 26 | 23 | 28/6,5 | 29,1/9,0 | sufE-like protein 2, chloroplastic isoform X2 (predicted) | Solyc05g013710.2 | 50 | 29,9 | 91 |
| 7603 | cytochrome P450 85A1 | CYP85A1 | A. thaliana | 28 | 34 | 55/6,5 | 54,1/9,1 | cytochrome P450 85A1 | Solyc02g089160.2 | 70 | 53,5 | 96 |
| 7605 | mannan endo-1,4-beta-mannosidase 3 | Solyc12g013750.1 | S. Lycopersicum | 34 | 24 | 48/6,5 | 45,3/5,6 | |||||
| 7701 | linoleate 9S-lipoxygenase B | Solyc01g099190.2.1 | S. Lycopersicum | 74 | 22 | 60/6,4 | 97,5/5,6 | |||||
| 7702 | argininosuccinate lyase, chloroplastic | At5g10920 | A. thaliana | 28 | 23 | 60/6,4 | 57,9/5,6 | argininosuccinate lyase | Solyc04g076320.2 | 80 | nd | 89 |
| 7703 | F-box/WD-40 repeat-containing protein At5g21040 | At5g21040 | A. thaliana | 40 | 33 | 66/6,7 | 61,1/8,5 | F-box/WD-40 repeat-containing protein At5g21040-like (predicted) | Solyc06g073650.1 | 59 | 60,9 | 100 |
| 7704 | zinc finger CCCH domain-containing protein 46 | At3g51950 | A. thaliana | 29 | 23 | 60/6,5 | 60,2/6,5 | zinc finger CCCH domain-containing protein 46-like isoform X2 (predicted) | Solyc01g008600.2 | 46 | 61,9 | 79 |
| 7705 | pentatricopeptide repeat-containing protein At1g15480, mitochondrial | At1g15480 | A. thaliana | 29 | 17 | 66/6,4 | 67,8/6,4 | pentatricopeptide repeat-containing protein At1g80270, mitochondrial (predicted) | Solyc12g020050.1 | 77 | 70,1 | 59 |
| 8107 | growth-regulating factor 10 | GRF10 | O. sativa | 43 | 16 | 22/6,8 | 22,5/9,0 | growth-regulating factor 9-like (predicted) | Solyc08g079800.2 | 59 | 24,6 | 51 |
| 8205 | 40S ribosomal protein S3a-2 | RPS3AB | A. thaliana | 29 | 35 | 28/6,7 | 29,9/9,7 | 40S ribosomal protein S3a (predicted) | Solyc06g072490.2 | 84 | 29,6 | 92 |
| 8304 | aldo-keto reductase family 4 member C11 | AKR4C11 | A. thaliana | 37 | 18 | 35/6,6 | 35,2/6,5 | aldo-keto reductase family 4 member C9 (predicted) | Solyc00g015750.1 | 78 | 35,0 | 100 |
Proteins abundance varied in response tothe As and/or As + Si treatments. Notes: a spot number assigned following 2DE separation, b putative protein identification, c encoding gene of the closest match in the Swiss-Prot database, d organism, e score, f percentage of coverage of the matching peptide sequence tags derived from MASCOT analysis, g estimated mass and pI, h predicted mass and pI of the closest match, i best BLAST hit in tomato, l gene name, m predicted mass, n % identity, o % coverage identified by BLASTp search of tomato