Figure 3. Cleavage of fXI-Ala⁵⁵⁷ by fXIIa or thrombin in the presence of nucleic acids.

(Top Row) SDS-PAGE of fXI-Ala557 (200 nM) incubated with vehicle (control), 30 μg/ml DNA, or 5 μg/ml RNA (A) without a protease, (B) with 15 nM thrombin, or (C) with 15 nM fXIIa. Incubation times in minutes are shown at the tops of gels. Abbreviations: XI – zymogen fXI, HC – fXIa heavy chain, LC – fXIa light chain. (Bottom Row) Reduction of the 80 kDa fXI-Ala557 zymogen band on the gels immediately above each graph, as determined by densitometry. Results are for reactions without nucleic acid (○), with DNA (□), or with RNA (∆).