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. Author manuscript; available in PMC: 2018 Apr 3.
Published in final edited form as: Thromb Haemost. 2017 Jan 26;117(4):671–681. doi: 10.1160/TH16-09-0691

Figure 5. Effects of HK and HKa on fXI activation in the presence of nucleic acids.

Figure 5

(A) Plasma fXI (30 nM) was incubated with vehicle (○,●), 5 μg/ml DNA (□,■), or 1 μg/ml RNA (∆,▲) in the absence (○,□,∆) or presence (●,■,▲) of 30 nM HK. Reactions included no activating protease (No Protease), 2.5 nM fXIIa or 2.5 nM thrombin. At indicated time points, aliquots were tested for fXIa activity by chromogenic assay. (B) Western blot for HK using samples from the reactions containing HK and DNA shown in panel A. Positions of standard for HK and HKa are indicated at the right of the images. (C) Same for panel A except that HK was replaced with 30 nM HKa. For panels A and C error bars are +/−one SD.