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. 2017 Nov 21;16:207. doi: 10.1186/s12934-017-0825-3

Fig. 5.

Fig. 5

Construction of the T. reesei egl2 overexpression strains. a Cassettes used for egl2 overexpression in the uracil auxotrophic strain QP4. b The CMC agar plate used to screen for the egl2 overexpression transformants. c PCR confirmation of the egl2- overexpression transformant QPE36, which shows an about 600-bp DNA fragment product using the primers Y-egl2-F1 and Y-PyrG-R1