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. 2017 Oct;12(10):1648–1654. doi: 10.4103/1673-5374.217345

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Copyright: © Neural Regeneration Research

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Percentage of positively stained area for growth associated protein 43 (GAP-43) in hippocampal neurons of postnatal day 21 weaning rats.

(A–D) Immunoreactivity for GAP-43 in the control and acrylamide (ACR) 5, 10 and 20 mg/kg groups. Scale bars: 100 μm. (a–d) Higher magnification for boxes in A–D. Scale bars: 10 μm. Positively stained cells (brown dots) showing normal morphology and function of neurons in the control group. Note that neurons in the ACR groups showed lighter staining and reduced GAP-43 numbers. The histogram in (E) shows the percentage of positively stained area (%). *P < 0.05, **P < 0.01, vs. control group (mean ± SD, n = 8, one-way analysis of variance followed by Tukey post hoc test).