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. 2017 Oct;12(10):1648–1654. doi: 10.4103/1673-5374.217345

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Copyright: © Neural Regeneration Research

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Percentage of synaptophysin (SYP)-immunoreactive area of hippocampal neurons in postnatal day 21 weaning rats.

(A–D) Immunoreactivity of SYP in the control and acrylamide (ACR) 5, 10 and 20 mg/kg groups. Scale bars: 100 μm. (a–d) Higher magnification for boxes in A–D. Scale bars: 10 μm. Brown points represent positive staining, demonstrating that ACR had a mild toxic effect on SYP volume. (E) Percentage of positively stained SYP area in postnatal day 21 weaning rats in hippocampal neurons. *P < 0.05, vs. control group (mean ± SD, n = 8, one-way analysis of variance followed by Tukey post hoc test).