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Western blot assay for synaptophysin (SYP) in the hippocampal dentate gyrus of postnatal day 21 weaning rats.
The gray value ratio of SYP to β-tubulin in each group was measured. The ratio in the control group was set as a reference, and all experimental ratios were compared with the control group. SYP expression was decreased following acrylamide (ACR) exposure. *P < 0.05, **P < 0.01, vs. control group (mean ± SD, n = 8, one-way analysis of variance followed by Tukey post hoc test).
