Fig. 7.

Expression of collagen fibers, MMP-2, 9 and Hh pathway components in BALB/c athymic nude mice. a, b, Collagen fiber structure in tumors from untreated mice (control) or mice treated with DOX alone, LIUS alone or DOX + LIUS was visualized by Masson’s trichrome staining (^# p < 0.05). The expression of Shh, Gli-1, MMP-2 and MMP-9 in subcutaneous tumors was evaluated by immunohistochemical staining (c, d, magnification 400×) and Western blot analysis (e, f). β-actin was assessed as an internal control. Representative data from three independent experiments are shown. Data are presented as the mean ± SD (n = 3). * p < 0.05 versus control