Figure 7.
PIMT increases PPARγ- and RXRα-mediated transactivation of reporter expression in CV-1 cells. (A) CV-1 cells were cotransfected with 1.5 μg of reporter construct PPRE-TK-LUC, 25 ng of PCMV-mPPARγ, and 0.4 μg of PCMVβ, along with the indicated plasmid in the absence (solid bar) or presence (open bar) of 10−5 M BRL49653. Transfection, without the indicated plasmid, was compensated by adding the same amount of PCDNA3.1. The activity obtained on transfection of PPRE-TK-LUC, without exogenous PIMT in the absence of ligand was taken as 1. Results are the mean of three independent transfections. (B) Transfection analysis for RXRα was performed in the same way as PPARγ, except for using RXRE-TK-LUC and PCMX-RXRα in the absence (solid bar) and presence (hatched bar) of ligand 9-cis-retinoic acid.