Table 1.
Identification of Trichinella pseudospiralis excretory-secretory proteins by MALDI-TOF/TOF-MS/MS. All the MASCOT scores are >23 (P < 0.05)
| No. | MSa | MPb | SCc | Mw(KDa)/pId | Mw(KDa)/pIe | UniProt-NCBInr ID | Description |
|---|---|---|---|---|---|---|---|
| 1 | 71 | 12 | 37 | 38.6/6.52 | 44.9/5.83 | Q32R66_TRISP | Adult-specific DNase II-10 |
| 2 | 216 | 19 | 35 | 50.9/6.15 | 43.9/5.95 | KRX96478.1 | Enolase |
| 3 | 83 | 11 | 13 | 110.6/9.40 | 47.2/5.74 | KRY76695.1 | Serine/threonine-protein kinase ULK3 |
| 4 | – | – | – | – | – | – | Not identified |
| 5 | 110 | 10 | 17 | 49.6/6.69 | 35.5/5.30 | G4XSU5_TRISP | Poly-cysteine and histidine tailed protein isoform 2 |
| 6 | – | – | – | – | – | – | Not identified |
| 7 | 232 | 10 | 15 | 49.6/6.69 | 29.3/6.05 | G4XSU5_TRISP | Poly-cysteine and histidine tailed protein isoform 2 |
| 8 | 37 | 1 | 2 | 50.7/9.20 | 23.1/6.19 | E5SVR8_TRISP | Putative venom allergen 5 |
| 9 | 40 | 1 | 2 | 47.6/8.98 | 23.0/6.23 | KRY01512.1 | Chymotrypsin-like elastase family member 1 |
| 10 | 279 | 9 | 40 | 28.7/8.33 | 34.6/6.14 | E5SZQ3_TRISP | Deoxyribonuclease II superfamily |
| 11 | – | – | – | – | – | – | Not identified |
| 12 | 577 | 15 | 49 | 38.6/6.52 | 31.1/5.91 | Q32R66_TRISP | Adult-specific DNase II-10 |
| 13 | 326 | 12 | 23 | 49.6/6.69 | 24.2/6.31 | G4XSU5_TRISP | Poly-cysteine and histidine tailed protein isoform 2 |
| 14 | – | – | – | – | – | – | Not identified |
| 15 | 152 | 15 | 26 | 72.8/8.83 | 35.4/6.54 | Q9BJM1_TRISP | Serine protease |
| 16 | 266 | 13 | 23 | 49.6/6.69 | 28.9/6.77 | G4XSU5_TRISP | Poly-cysteine and histidine tailed protein isoform 2 |
| 17 | 180 | 12 | 21 | 72.8/8.83 | 29.4/6.53 | Q9BJM1_TRISP | Serine protease |
| 18 | 79 | 11 | 32 | 41.5/8.45 | 33.0/5.37 | KRZ27934.1 | Golgin-45 |
| 19 | 80 | 12 | 10 | 110.6/9.40 | 32.7/5.50 | KRY76695.1 | Serine/threonine-protein kinase ULK3 |
| 20 | 66 | 10 | 42 | 30.9/9.20 | 122.2/5.29 | KRY64535.1 | Uncharacterized protein |
| 21 | 225 | 12 | 35 | 38.6/6.52 | 122.6/5.51 | Q32R66_TRISP | Adult-specific DNase II-10 |
| 22 | 331 | 8 | 16 | 57.1/7.56 | 20.1/6.05 | KRY73124.1 | Peptidase inhibitor 16, partial |
| 23 | 606 | 19 | 61 | 38.6/6.52 | 33.0/6.67 | Q32R66_TRISP | Adult-specific DNase II-10 |
| 24 | 124 | 20 | 30 | 72.8/8.83 | 34.9/6.73 | Q9BJM1_TRISP | Serine protease |
| 25 | 68 | 5 | 82 | 4.97/6.55 | 34.9/5.19 | E5SL33_TRISP | Death-associated protein 1 |
| 26 | 318 | 12 | 21 | 49.6/6.69 | 32.9/5.12 | G4XSU5_TRISP | Poly-cysteine and histidine tailed protein isoform 2 |
| 27 | 175 | 11 | 33 | 38.6/6.52 | 62.7/5.55 | Q32R66_TRISP | Adult-specific DNase II-10 |
| 28 | 82 | 9 | 9 | 110.6/9.40 | 25.9/5.36 | KRY76695.1 | Serine/threonine-protein kinase ULK3 |
aMascot score
bMatched peptide
cSequence coverage (%)
dTheoretical
eExperimental