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Determination of iNOS activity (A), oxygenase domain activity (B), and cytochrome c reductase activity (C) of human iNOS and iNOS D280A mutant. NO production was evaluated by using HbO2 capture assay. Oxygenase domain activity was assessed by measuring the H2O2-supported oxidation of Nω-hydroxy-l-Arg. Data are mean ± SD of three experiments.
