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. 2017 Sep 1;31(6):1730–1739. doi: 10.1111/jvim.14812

Figure 1.

Figure 1

Phenotypes of PBLs in dogs with IBD (left) and healthy control dogs (right). Both columns are representative examples of the respective dog groups. Data are presented by histograms showing the expressions of 1 parameter per histogram and by contour plots showing percentages of cells with various properties. Top row: Representative dot blot showing the forward/sideward scatter (FSC/SSC) properties of the analyzed PBLs gating the lymphocyte population (red). Rows 2 #bib4, and 6 show representative histograms of CD45, TCRαβ, and CD8β expressions with negative cells on the left and positive cells on the right side of the graphs. The percentages of the respective positive populations are indicated by the numbers in the right upper corners of the graphs. Row 3 shows representative contour plots of gated cells labeled with mAb against CD3 and CD79. CD3+ cells are displayed in the upper left corner; CD79αcγ+ cells are displayed in the lower right corner of the graphs. The percentages of the respective positive populations are indicated by the adjacent numbers. Row 5 shows representative contour plots of gated cells labeled with mAb against CD8α and CD4. CD8α+ cells are displayed in the upper left corner, and CD4+ cells are displayed in the lower right corner of the contour plot. There is a small population of double‐positive cells (CD4+ CD8+) displayed in the right upper corner of the graphs. The percentages of the respective positive populations are indicated by the adjacent numbers.