Table 1.
List of canine‐specific and anti‐human cross‐reactive mAbs used for FCM analysis of canine PBLs
| mAb | Clone | Isotype | Fluorescence Labeling | Target Species/Species Cross‐Reactivitya |
|---|---|---|---|---|
| CD45 | YKIX716.13 | rIgG2b | APC | Anti‐canine or Antihuman |
| CD3 | CD3‐12 | rIgG1 | FITC | Anti‐humana |
| CD4 | YKIX302.9 | rIgG2a | APC | Anticanine |
| CD8α | YCATE 55.9 | rIgG1 | PE | Anticanine |
| CD8β | CA15.4G2 | mIgG1 | α‐mIgG1‐AlexaFluor647b , c | Anticanine |
| CD21 | B‐ly‐4 | mIgG1 | APC | Anti‐human37 |
| CD79αcy | HM57 | mIgG1 | PE | Anti‐human38 |
| TCRαβ | CA15.8G7 | mIgG1 | α‐mIgG1‐AlexaFluor488b , d | Anticanine |
| TCRγδ | CA20.8H1 | mIgG2a | α‐mIgG2a‐FITCb , e | Anticanine |
mAb, monoclonal antibodies; CD, cluster of differentiation; IgG, immunoglobulin G; TCR, T‐cell receptor; m, mouse; α‐m, anti‐mouse; r, rat; FITC, fluorescein isothiocyanate; APC, allophycocyanin; PE, phycoerythrin; a, cross‐reactivity established by: CD3‐12, Serotec, technical datasheet MCA1477, CD21, see reference 37, CD79, see reference 38; b, fluorescence labeling was achieved by use of a secondary antibody; c, goat anti‐mouse IgG1‐Alexa647; Life Technologies, Carlsbad, CA; d, goat anti‐mouse IgG1‐Alexa488; Life Technologies, Carlsbad, CA; e, goat F(ab)2 anti‐mouse IgG2a‐FITC; SouthernBiotech, Birmingham, AL.