Table 2.
Differential counts of bone marrow cells from wild-type and transgenic mice
| Mouse type | Differential counts, %
|
||||||
|---|---|---|---|---|---|---|---|
| Myeloblasts + promyelocytes | Metamyelocytes + myelocytes | Neutrophils | Eosinophils | Lymphoid | Monocytes | Erythroid | |
| WT (n = 3) | 7.0 ± 2.0 | 13.7 ± 4.5 | 34.7 ± 5.1 | 1.3 ± 0.6 | 12.3 ± 6.5 | 3.0 ± 1.0 | 28.0 ± 16.1 |
| WT-ENU (n = 4) | 0 | 0.7 ± 1.2 | 1.9 ± 1.0 | 0 | 94.7 ± 1.5 | 0 | 2.7 ± 1.9 |
| Tg-ENU-ALL (n = 3) | 0 | 2.5 ± 3.5 | 7.0 ± 4.9 | 0 | 83.5 ± 12.0 | 2.5 ± 0.7 | 4.5 ± 0.7 |
| Tg-ENU-AML (n = 5) | 42.2 ± 4.4 | 16.5 ± 2.6 | 15.7 ± 3.8 | 3.6 ± 2.4 | 7.3 ± 3.8 | 2.8 ± 1.6 | 15.5 ± 8.0 |
Wild-type (WT) and transgenic (Tg) mice treated with ENU were killed when they were moribund. Bone marrow cells were harvested and cytospun for Wright–Giemsa staining. Differential counts were performed under a microscope. Normal wild-type mice were used as negative controls. The data reported here are means ± SD. In each bone marrow sample, 200 cells were counted.