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. 2017 Nov 21;4:170170. doi: 10.1038/sdata.2017.170

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

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(a,b) A fluorescent spheroid from an alginate-BR culture with (b) the detection of EdU positive cell illustrated, where red circles indicate positive cells. (c) A small subset of an image-stack of a matrix (Matrigel, DMSO) embedded 3D spheroid culture, where in panel (d) the detection of EdU positive cells is shown as circles within spheroids (red circles outside the spheroid originate from proliferating fibroblasts). The spheroids themselves are approximated by ellipsoids and shown in color. In (e,f) the top and side view of the segmentation contours are shown to illustrate the ellipsoid approximation. Spheroids only visible in (f) but not in (e) are originating from (x,y)-coordinates outside the subset of the xy-plane. RFP (red): Tumor cells; GFP (green): Stromal fibroblasts; DAPI (blue): Nuclei; EdU (pink): Proliferating nuclei.