Table 2. Experimental 2D/3D cell culture conditions used in the PREDECT study.
|
2D |
3D floater |
3D embedded |
3D bioreactor |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TC (/well) | TC:SC | FBS (%) | TC (/well) | TC:SC | FBS (%) | TC (/well) | TC:SC | FBS (%) | TC (/ml) | TC:SC | FBS (%) | Medium | |
| TC: tumor cells; SC: stromal cells. |
|||||||||||||
| Breast | 6,000 | 03:01 | 10 | 1,000 | 03:01 | 10 | 10,000 | 10:01 | 2 | 20,000 | 01:01 | 10 | DMEM |
| Prostate | 10,000 | 10:01 | 2 | 1,000 | 10:01 | 2 | 10,000 | 10:01 | 2 | N/A | N/A | N/A | RPMI |
| Lung | 10,000 | 10:01 | 10 | 400/200* | 01:01 | 10 | 10,000 | 10:01 | 2 | 20,000 | 01:01 | 10 | RPMI |
*mono/co-cultures; N/A: Not applicable (not performed). Taken from ref. 1.