Table 5. Microscopes, Objectives, and filters for imaging.

Fluorescence Microscope settings
Platform	Instrument	Objective (NA)	Fluoro-phore	Filters
				Excitation [nm]	Emission [nm]
NA—Numerical aperture; BP—Band pass; DPSS—Diode-pumped solid-state lasers; OPSL CW—Optically pumped semiconductor lasers continuous wave. Taken, with modifications, from ref. 5.
3D floaters	Zeiss (LSM700)	10x EC PLAN NEOFLUAR M27 (0.3)	dTomato	BP 546/12	BP 575–640
			GFP	BP 450–490	BP 515–565
			Hoechst	365	BP 445/50
			EdU	BP 640/30	BP 690/50
	Zeiss (LSM510 META Multiphoton)	10x Plan Apochromat (0.45)	tRFP	561	BP 565–615
			GFP	488	BP 500–530
			Hoechst	405	BP 390–465
			EdU	640	BP 650–710
	ZEISS (Axiovert 200M)	10x A-Plan (0.25)	dTomato	BP 546/12	BP 575–640
			GFP	BP 475/40	BP 530/50
3D BR	Andor (Revolution WD Spinning Disk)	10x Plan Fluor (0.3) 20x Plan Fluor (0.75)	dTomato	561 (DPSS)	BP 595/50
			GFP	488 (OPSL CW)	BP 525/50
			Hoechst	405	BP 460/50
			EdU	640 (DPSS)	BP 685/40
3D matrix	Zeiss (LSM700)	10x EC PLAN NEOFLUAR M27 (0.3)	dTomato	BP 546/12	BP 575–640
			GFP	BP 450–490	BP 515–565
			Hoechst	365	BP 445/50
			EdU	BP 640/30	BP 690/50
	Yokogawa (CV 7,000 Spinning Disk)	10x UPLSAPO (0.4)	dTomato	561	BP 600/37
			GFP	488	BP 522/35
			Hoechst	405	BP 447/45
			EdU	640	BP 676/29
	Zeiss (Axiovert 200M)	10x A-Plan (0.25)	tRFP	BP 546/12	BP 575–640
			GFP	BP 450–490	BP 515–565
			Hoechst	365	BP 445/50
			EdU	BP 640/30	BP 690/50