Fig. 1.
MOG35–55-EAE induction elicits a humoral response directed towards the spinal cord and NPCs. a Mean clinical score of all animals during EAE course. Error bars show the standard statistical error of the mean (SEM). b XTT assay indicated the relative NPC viability % of NPCs treated with purified IgG from EAE-AS and unpurified EAE-AS compared with NPCs treated with NAIVE-AS (control), in three different concentrations (0.1, 1, and 10 μg/ml). Data are presented as relative viability percentage (%) as mean ± SEM. Western blot of various antisera from animals immunized with MOG (EAE-AS) yielded one band approximately at 30 kDa on spinal cord substrate (c) and bands at above 60 kDa, above 40 kDa, and around 30 kDa on NPC substrate (d). Lane probed with EAE-AS demonstrates a representative antiserum. Anti-MOG antibody and anti-actin-loading control were also used