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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Mol Cancer Res. 2017 May 23;15(8):1063–1072. doi: 10.1158/1541-7786.MCR-17-0062

Figure 4. 2-ME activates Aurora A Kinase in cells arrested in S phase.

Figure 4

(A) Flow cytometry analysis of unsynchronized LNCaP and C4-2 treated with 2-ME for 24h (upper left). Immunoblots for indicated proteins and tubulin as a control (upper right). (B) LNCaP or C4-2 cells were held in G1/S by adding aphidicholin for 24 h. Cells were treated with or without 2-ME (2 μM) for additional 24 h in continued presence of aphidicholin. DNA content of LNCaP and C4-2 (Lower left) and immunoblotted for indicated proteins and tubulin serves as control (lower right)