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Testosterone hydroxylation by recombinant CYP3A4, CYP3A5, and CYP3A7 enzymes. Representative MRM chromatograms for hydroxylated testosterone metabolites (MRM transitions: 305 > 269 atomic mass unit) formed in incubations of testosterone (50 µM) with CYP3A4 (A), CYP3A5 (B), and CYP3A7 (C) Supersomes for 10 minutes in the presence (continuous black trace) or absence (dotted gray trace) of the NADPH-generating system.&
