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. 2017;18(8):2249–2254. doi: 10.22034/APJCP.2017.18.8.2249

Figure 3.

Figure 3

Dot Blotting (A) and western blotting (B) analysis results. A: The lane 1: Positive control (t-PA standard, Actylase); lane 2-4 related to supernatants of time points 96 (4 days), 120 (5 days), and 144 (6 days) respectively; lane 5: Negative control (The supernatant of Pichia pastoris KM71H without t-PA gene). B: M: Protein marker; lane 1: Positive control (t-PA standard, Actylase); lane 2: the supernatant of recombinant Pichia pastoris KM71H; lane 3: Negative control (The supernatant of Pichia pastoris KM71H without t-PA gene).