Fig. 3.
Survival of BALB/c mice vaccinated with CPS-TetHc (acetylated and deacetylated CPS), TetHc and alum followed by challenge with 1.17 × 105 CFU via the IP route of B. pseudomallei K96243 (approximately 157 x median lethal doses (MLDs)). Mice were vaccinated three times in two-week intervals prior to challenge. Vaccination with acetylated CPS conjugate offered significantly greater protection than the deacetylated CPS conjugate [p = 0.0015 Log-Rank (Mantel Cox test [35])]. The deacetylated CPS-TetHc conjugate did not offer any significantly greater protection than adjuvant or TetHc alone [p = 0.1694 and p = 0.0872 Log-Rank, respectively (Mantel Cox test)].