Fig. 4.
ELISA analysis of acetylated and deacetylated CPS-specific IgG and IgM immune responses following vaccination with CPS conjugate vaccines. Mice were vaccinated with native CPS-TetHc or deacetylated CPS-TetHc three times at 2-week intervals. Serum was obtained from mice 14 days after the third vaccination, and titres of IgG and IgM specific for CPS or deacetylated CPS were determined by ELISA. Individual symbols represent a single immunised mouse. Significance was determined by unpaired t-test. LOD, limit of detection.