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. 2001 Aug 14;98(18):10422–10427. doi: 10.1073/pnas.181331398

Figure 2.

Figure 2

Separation of cryptococcal proteins by anion-exchange chromatography. Supernatants from glass bead-disrupted C. neoformans were separated on a Mono-Q column at pH 6 by using a NaCl gradient. One-milliliter fractions were collected and tested for their capacity to stimulate hybridoma P1D6 to produce IL-2 in the presence of APCs. Gray box demonstrates the fractions that stimulated IL-2 production. Chromatogram is representative of 10 experiments.