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. 2016 Sep 11;64(6):1750–1761. doi: 10.1111/tbed.12562

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© 2016 The Authors. Transboundary and Emerging Diseases Published by Blackwell Verlag GmbH

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Results of serological surveys performed on Italian and Spanish European brown hare sera tested for EBHSV and RHDV2. Hare sera were tested in cELISAs for EBHSV (■ black bars) and RHDV2 ( grey bars). Titre values are reported as reciprocals (i.e. 20 means 1/20). The categories of negative and doubtful sera were grouped together. Panels summarize the titre distributions of: (a) 149 sera collected in northern Italy between 2007 and 2010 (pre‐RHDV2 period); (b) 154 sera collected in northern Italy during 2012; (c) 253 sera collected in northern Italy during 2013; (d). 106 sera collected in Spain during the same period (2014) and in the Barcelona Province where the two EBHs were found dead.

Inline graphic — Results of serological surveys performed on Italian and Spanish European brown hare sera tested for EBHSV and RHDV2. Hare sera were tested in cELISAs for EBHSV (■ black bars) and RHDV2 ( grey bars). Titre values are reported as reciprocals (i.e. 20 means 1/20). The categories of negative and doubtful sera were grouped together. Panels summarize the titre distributions of: (a) 149 sera collected in northern Italy between 2007 and 2010 (pre‐RHDV2 period); (b) 154 sera collected in northern Italy during 2012; (c) 253 sera collected in northern Italy during 2013; (d). 106 sera collected in Spain during the same period (2014) and in the Barcelona Province where the two EBHs were found dead.