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. 2017 Feb 9;27(6):770–780. doi: 10.1111/bpa.12452

Table 1.

Antibodies and dilutions for standardized immunohistochemical staining procedure.

Antibody overview
Antibody Company Clone Dilution Pretreatment Method Incubation
CD3 Neomarker SP7 1:200 pH6 citrate buffer Autostainer 30 min room temp
CNPase Millipore 11‐5B 1:500 pH6 citrate buffer Coverplates Over night 4°C
MAP2 Millipore AP20 1:8000 pH6 citrate buffer Autostainer 30 min room temp
MBP Dako A0623 1:400 pH6 citrate buffer Autostainer 30 min room temp
MOBP Sigma HPA035152 1:100 pH9 Coverplates Over night 4°C
NeuN Millipore A60 1:2000 pH6 citrate buffer Autostainer 30 min room temp
NG2 Millipore AB5320 1:200 non Humidity chamber Over night 4°C
NogoA Millipore AB5888 1:1000 pH6 citrate buffer Coverplates Over night 4°C
Olig2 IBL 18953 1:100 pH6 citrate buffer Autostainer 60 min room temp
PDGFRa Abcam ab61219 1:50 non Humidity chamber Over night 4°C
pS6(Ser240/244) Cell Signaling D68F8 1:1000 pH6 citrate buffer Coverplates Over night 4°C
SMI32 Covance SMI‐32R 1:200 pH6 citrate buffer Autostainer 30 min room temp
Tppp7/p25 Clin. Center, Univ. Pecs mAb6C1 1:1000 pH6 citrate buffer Autostainer 30 min room temp
Vimentin Dako V9 1:50 non Autostainer 30 min room temp