Table 1.
Antibody overview | ||||||
---|---|---|---|---|---|---|
Antibody | Company | Clone | Dilution | Pretreatment | Method | Incubation |
CD3 | Neomarker | SP7 | 1:200 | pH6 citrate buffer | Autostainer | 30 min room temp |
CNPase | Millipore | 11‐5B | 1:500 | pH6 citrate buffer | Coverplates | Over night 4°C |
MAP2 | Millipore | AP20 | 1:8000 | pH6 citrate buffer | Autostainer | 30 min room temp |
MBP | Dako | A0623 | 1:400 | pH6 citrate buffer | Autostainer | 30 min room temp |
MOBP | Sigma | HPA035152 | 1:100 | pH9 | Coverplates | Over night 4°C |
NeuN | Millipore | A60 | 1:2000 | pH6 citrate buffer | Autostainer | 30 min room temp |
NG2 | Millipore | AB5320 | 1:200 | non | Humidity chamber | Over night 4°C |
NogoA | Millipore | AB5888 | 1:1000 | pH6 citrate buffer | Coverplates | Over night 4°C |
Olig2 | IBL | 18953 | 1:100 | pH6 citrate buffer | Autostainer | 60 min room temp |
PDGFRa | Abcam | ab61219 | 1:50 | non | Humidity chamber | Over night 4°C |
pS6(Ser240/244) | Cell Signaling | D68F8 | 1:1000 | pH6 citrate buffer | Coverplates | Over night 4°C |
SMI32 | Covance | SMI‐32R | 1:200 | pH6 citrate buffer | Autostainer | 30 min room temp |
Tppp7/p25 | Clin. Center, Univ. Pecs | mAb6C1 | 1:1000 | pH6 citrate buffer | Autostainer | 30 min room temp |
Vimentin | Dako | V9 | 1:50 | non | Autostainer | 30 min room temp |