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. 2017 Aug 8;18(9):1295–1312. doi: 10.1111/mpp.12564

Figure 5.

Figure 5

The expression of various categories of Fusarium graminearum genes previously associated with virulence. (A) The accumulative expression of transcripts encoding for putative secreted effectors with increased transcript abundance in either symptomless or symptomatic wheat tissue. (B) The accumulative expression of PHI‐base genes, and those previously defined as being pathogenicity associated, during in vitro culture and wheat infection. (C) The two‐step regulation of hydrolytic enzyme production by F. graminearum during wheat infection. The accumulative expression of 50 putative carbohydrate‐active enzyme (CAZyme) and six lipase encoding genes throughout in vitro culture and wheat infection. This revealed increased CAZyme and lipase transcript abundance in planta versus in vitro culture, and the highest accumulative CAZyme abundance within symptomatic wheat tissue. (D) The expression profiles of four characterized CAZymes, which have been proven to contribute to virulence (Urban et al., 2015), and two characterized lipases peak in symptomatic wheat tissue. CM, complete medium; MM‐C, minimal medium without carbon; MM‐N, minimal medium without nitrogen (Gueldener et al., 2006).