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. 2017 Aug 16;37(12):1420–1427. doi: 10.1002/jat.3510

Figure 4.

Figure 4

Amount of DNA SBs in V79 cells analyzed with the alkaline DNA unwinding technique after 2 h exposure to Ni nanoparticles in air–liquid interface either with or without 4ANI. The exposure concentration of Ni in this assay is estimated to be approximately between 0.3 and 0.4 μg cm−2. Each bar represents the mean value of three independent experiments. Error bars represent ±SEM. *statistically significant values (P < .05). 4ANI, 4‐amino‐1,8‐naphtalimide; SBs, strand brakes