. Author manuscript; available in PMC: 2018 Aug 1.

Published in final edited form as: Exp Eye Res. 2017 May 30;161:17–29. doi: 10.1016/j.exer.2017.05.011

Table 2.

Sodium borate buffer

150 mM Sodium Borate-saline buffer, for diluting poly-L-ornithine solution
Chemical	g/500 ml	Catalogue number (Sigma-Aldrich)
Boric acid (H₃BO₃)	4.05	B6768
Sodium tetraborate, anhydrous (Borax; Na₂B₄O₇)	3.6	221732
Sodium chloride (NaCl)	2.2	S5886

1. Add solid reagents to ~450 ml deionized (di) water; stir at room temperature to effect solution.

2. Adjust pH to 8.4 – 8.5, if necessary, with 0.1 N NaOH or 0.1 N HCl; then bring final volume to 500 ml with di water.

3. Filter-sterilize using vacuum, store indefinitely in refrigerator.

4. Handle using sterile technique in biosafety cabinet.