Skip to main content
. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Exp Eye Res. 2017 May 30;161:17–29. doi: 10.1016/j.exer.2017.05.011

Table 4.

Balanced Saline Solutions*
Modified Hanks’ Balanced Saline Modified Earle’s Balanced Saline
Component Source Catalogue Number mmol/L mmol/L Comments
NaCl Sigma-Aldrich, Saint Louis, MO S5886 138.5 126.5 May be prepared as combined 20x monovalent stock with KCl and sodium dihydrogen phosphate
KCl Sigma-Aldrich P5405     3.5     3.5
NaH2PO4•H2O Sigma-Aldrich 71507     1     1
CaCl2•2H2O Sigma-Aldrich C7902     0.5     0.5 Calcium and magnesium salts may be prepared as a combined 20x divalent stock1
MgCl2•6H2O Sigma-Aldrich M2393     0.27     0.27
MgSO4•7H2O Sigma-Aldrich 63138     0.37     0.37
HEPES2 Sigma-Aldrich H0887   15   15 Supplied as 1 M sterile solution; store refrigerated, protected from light
NaHCO3 Sigma-Aldrich S5761     4.2   15
D-Glucose Sigma-Aldrich G7021     4.15     4.15
D-Fructose Sigma-Aldrich F3510     4.15     4.15
*

Adjusted pH = 7.2 – 7.4, approximately 300 mOsm; filter sterilize and store refrigerated

1

Leave out calcium and magnesium salts for Ca+2, Mg+2-free balanced salt solutions

2

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid