Table 5.

Incubation medium (IM) for experimental treatment of retinal cell lines^*

Component	Catalogue number (all Sigma-Aldrich)	Concentration (or proportional volume) per 100 ml final volume	Comments
DMEM with HEPES	D6171	47.5 ml	High glucose formulation
Ham’s F-12 with HEPES	N8641	47.5 ml
TAPSO	T9269	100 mg	Solid stored at room temperature (rt)
Hydrochloric acid	H9892	0.2 ml	Supplied as sterile 1 N solution; store at rt
Transferrin	T8158	0.75 mg	Solid and stock aliquots both stored at −20°C
Sodium pyruvate	S8636	1 ml	Supplied as sterile 100 mM solution; refrigerate
Non-essential amino acids	M7145	0.5 ml	Supplied as sterile solution; refrigerate
Glucose	G7021	37.5 mg	Supplemental
Fructose	F3510	37.5 mg
Alanyl-glutamine	A8185	43.4 mg	Solid stored at 4°C; Stock aliquots stored at −20°C
Triiodothyronine	T6397	0.35 µg	Solid stored at −20°C; Stock aliquots stored refrigerated
Hydrocortisone	H0396	0.6 µg	Supplied in powder form as soluble complex with hydroxypropyl-β-cyclodextrin; amount given with respect to hydrocortisone; store powder desiccated at −20°C

^*Use with pCO₂ = 6%