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Membrane depolarization assay. Flow cytometry-based analysis of MSSA cells treated with DiOC2(3) dye control (A), ecteinamycin (B), and CCCP (C). (D) The ratio of red to green fluorescence for MSSA cells treated with DiOC2(3) dye and the corresponding compound. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) was used as a positive control. CCCP is a protonophore that disrupts membrane potential.50,51
