Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Best fits for kcat were obtained by simplex fitting, and subsequently fixed for nonlinear least squares analysis of Km, using the integrated Michaelis-Menten equation. kcat/Km values were calculated from the fits.
| No Aptamer | Sel-3 Control | 11.16 | 12.7 | |
|---|---|---|---|---|
| Km(μM)* | 0.26 ± 0.04 | 0.60 ± 0.09 | 3.1 ± 0.1 | 2.9 ± 0.4 |
| kcat (min-1)* | 12 | 5.4 | 3.7 | 3.7 |
| kcat / Km (μM-1min-1) | 46 | 9 | 1.2 | 1.3 |
