Fig 3. DNA and RNA extraction from plant and animal pathogens using cellulose-based paper.

(A) DNA was purified from P. syringae-infected Arabidopsis leaves at different stages of infection using cellulose discs and a fragment of the P. syringae genome amplified by PCR. The band intensities achieved with cellulose discs relative to the positive control appear below each band. (B) A Whatman No.1 disc was used to purify DNA from a lung swab of a pig infected with A. pleuropneumoniae that had been placed in extraction buffer (see Material and methods). As a control, 1 μl of the raw lysate was added directly into a separate PCR reaction. (C) Whatman No.1 discs were used to purify nucleic acids from tomato plants infected with cucumber mosaic virus. The cellulose discs were added to RPA reactions with or without the presence of RT. NTCs involved adding 1 μl of water instead of DNA template. (D) Cellulose discs were used to purify nucleic acids from tomato plants that were either healthy or infected with cucumber mosaic virus and subsequently amplify them in a LAMP isothermal reaction. LAMP, loop-mediated amplification; NTC, no template control; RPA, recombinase polymerase amplification; RT, reverse transcriptase.