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Middle-aged obese men each performed two separate trials (rest and exercise) 1–3 weeks apart. Rest trial muscle biopsies were taken at baseline (Base-REST) and post 2 hour hyperinsulinemic-euglycemic insulin clamp (Clamp-REST); exercise trial biopsies were obtained at baseline (Base-EX), 1 hour post-exercise (Post-EX) and after a subsequent 2 hour hyperinsulinemic-euglycemic insulin clamp (Clamp-EX).
