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. 2017 Nov 21;12(11):e0188458. doi: 10.1371/journal.pone.0188458

Fig 5. Effect of deletion mutants on resistant phenotype.

Fig 5

(A) Inoculation results of PATL9:ATL9, PATL9:ATL9ΔTM, PATL9:ATL9ΔPEST and PATL9:ATL9ΔRING transgenic plants. (B) Microscopic disease symptoms of PATL9:ATL9, PATL9:ATL9ΔTM, PATL9:ATL9ΔPEST and PATL9:ATL9ΔRING transgenic plants inoculated with G. cichoracearum. Scale bar is 1mm (C) Inoculation result of 35S:ATL9, 35S:ATL9ΔTM, 35S:ATL9ΔPEST and 35S:ATL9ΔRING overexpression plants. (D) Microscopic disease symptoms of 35S:ATL9, 35S:ATL9ΔTM, 35S:ATL9ΔPEST and 35S:ATL9ΔRING overexpression transgenic plants inoculated with G. cichoracearum. Scale bar is 1mm. All transgenic lines used in this experiment are in the atl9 mutant background. Four-week old Arabidopsis were inoculated with powdery mildew and placed in a growth chamber at 22°C day/19°C night with 16 hours of light per 24 hrs. Leaves at 5 dpi were harvested and stained in a trypan blue solution overnight and then decolorized in 95% ethanol overnight. The number of conidiophores per colony was determined from at least 18 leaves taken from 18 plants per genotype. Significance among different samples was analyzed by ANOVA and post hoc test. * indicates p-value < 0.05, when sample compared to Col-0. ** indicates p-value < 0.008 (Bonferroni adjustment p-value), when sample compared to Col-0.