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. 2017 Nov 21;12(11):e0188170. doi: 10.1371/journal.pone.0188170

Fig 1. Construction of DENPADS plasmids and characterization of the sensor module.

Fig 1

(A) pSen-Cre and pRep-EGFP were constructed as the sensor and reporter modules, respectively. The cleavage site of the DENV NS2B3 protease is indicated. BHK-21 cells were transfected with pSen-Cre in the presence or absence of pNS2B3. Localization of Cre (red) and (B) ER membranes (calnexin, green) or (C) NS3 (green) were detected by immunostaining. Nuclei were counterstained with Hoechst (blue). The fluorescence intensity was analyzed by LEICA Application Suite software (magnification, 100×). Cells transfected with pCre served as controls.