Skip to main content
. 2017 Nov 21;12(11):e0188170. doi: 10.1371/journal.pone.0188170

Fig 5. Determination of DENV titers by H-DENPADS.

Fig 5

(A) pRep-mHRP was constructed as a membrane-targeted HRP reporter module. (B) H-DENPADS cells were infected with DENV-2 for 48 hours. The expression of mHRP was examined by surface staining with anti-HRP antibodies using flow cytometry. (C) The function of mHRP was evaluated by color development after adding TMB substrate. (D) H-DENPADS cells were seeded in 96-well plates. After incubation for 24 hours, cells were infected with a 10-fold serial dilution of DENV-1, -2, -3, or -4. TMB substrate was added 24 hours after infection. The OD450 nm was examined with a spectrophotometer. The data are representative of 3 different experiments performed in hexaplicate. The dotted lines indicate the mean plus a 2-fold standard deviation of the non-infection group. (E) Virus titers of 43 DENV samples were converted by the standard curve, which was obtained from Fig 5D. Virus titers were determined in parallel by an immuno-focus assay. The correlation between the two methods is shown.