Fig 6. H-DENPADS as an anti-DENV drug screening platform.

H-DENPADS cells were exposed to DENV-2 at an m.o.i. of 5 in serum-free medium for 3 hours. After infection, cells were cultured in culture medium with various concentrations of (A, C, E) 2’-C-methyladenosine (2-CM) or (B, D, F) mycophenolic acid (MPA) as indicated. Mock infected cells served as the controls. Virus titers in the culture medium after infection for 24 hours were determined by immuno-focus assays (A, B) or H-DENPADS (C, D). The cytotoxic effect was analyzed with MTS assays (E, F). The data are representative of 3 different experiments performed in hexaplicate.