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. 2017 Nov 21;12(11):e0187560. doi: 10.1371/journal.pone.0187560

Fig 1. Effects of nutrients and hormones on SOAT1 mRNA accumulation in EECs and hepatocytes.

Fig 1

(A) The mRNA accumulations of SOAT1 during embryogenesis in YSM of Japanese quail. (B-I) EECs and hepatocytes were treated for 24 hours with different concentrations of a fatty acid, OA (oleic acid) or PA (palmitic acid); L(low) = 10 μM; H (high) = 100 μM, or glucose (3.3, 5.5 or 17.5 mM), or hormones (estrogen L = 1.8 nM, H = 18 nM; Dex L = 1 μm, H = 10 μm, glucagon L = 10 nM, H = 100 nM; IBMX L = 0.05 mM, H = 0.5 mM). SOAT1 mRNA accumulation was measured by Real-Time PCR and normalized to β-actin mRNA accumulation. Data were expressed as mean ± S.E.M. (n = 6) Statistical significance was determined by one-way analysis of variance. Tukey‘s test was used to evaluate differences between means. Control value was set as 1. Different letters indicate a significant difference (P≤0.05).