Fig 2. Determination of the region of MF6p/FhHDM-1 recognized by mAb MF6 in competitive ELISA.

ELISA plates coated with sMF6p/FhHDM-1 were incubated with mAb MF6 (diluted 1/200,000) previously incubated with twofold dilutions of the synthetic peptides sMF6p/FhHDM-1 (control for maximal inhibition; filled circles), sFhMF6c (residues ⁵⁶A-⁹⁰N; unfilled circles), sFhMF6c1 (residues ⁵⁶A-⁸¹T; filled triangles), sFhMF6c2 (residues ⁶⁵L-⁸⁸A; unfilled triangles), and sFhMF6a (residues ²³S-⁵⁵R; filled squares). Peptides sFhMF6c3 and sFhMF6c4 produced the same negative result as sFhMF6a, but for the sake of simplification were not represented here. Data are expressed as percentage inhibition of mAb MF6 by each peptide and are the mean values ± SD of duplicate wells.