Figure 2.

Demonstrating the application of the hspX’::GFP reporter strain in assessing and reporting on the localized induction of iNOS at the site of infection. PBS-immunized (naïve) and mice vaccinated with heat-killed Mycobacterium tuberculosis (vac) were infected with hspX’::GFP, smyc’::mCherry Erdman M. tuberculosis reporter strain. Fluorescence induction of the hspX promoter-dependent GFP is higher at 14 days in the vaccinated animals assessed by confocal microscopy of thick tissue sections (a), that were scored subsequently by Volocity (b). (c) The thick tissue sections were probed with antibodies against murine NOS2 (magenta) demonstrating the co-localization between GFP induction and NOS2 expression at the site(s) of infection. Data shown are detailed in Sukumar et al. [40].