Figure 6. mIFIH1^R mediates increased sensitivity to self-RNA ligands.

(a-e) Control or ADAR-null HEK293T cells were generated by lenti-CRISPR technology. Cell lines were transfected with 1 μg of mIFIH1 risk or non-risk constructs shown in Fig 2. Cells were analyzed by flow cytometry at 27 hours post-transfection for (a) geometric mean fluorescent intensity (MFI) and (b) percent GFP expression and combined data from four biological replicates are shown. (c-e) Quantitative RT-PCR for IFNB1 mRNA expression in control vs. ADAR-null cells transfected with mIFIH1^NR construct. (c) Representative data from one experiment. (d) Combined data from four biological replicates. (e) Relative levels of IFNB1 mRNA expression in mIFIH1^NR vs. mIFIH1^R transfected ADAR-null cells showing combined data from four biological replicates. Results were normalized by the Livak method as in Fig 2d. Statistical analysis performed using a two-tail student T test. Each data point represents one biological replicate. Error bars represent ± SEM. *p<0.05, **p<0.01.