. Author manuscript; available in PMC: 2018 Oct 1.

Published in final edited form as: Nat Protoc. 2017 Sep 7;12(10):2029–2049. doi: 10.1038/nprot.2017.079

TABLE 2.

Panel options for 1, 3/4, or 5 laser flow cytometers. Please see Reagents for recommended clone and purchasing information.

Fluorochrome	Panel A (1 laser)	Panel B (3/4 lasers)	Panel C (5 lasers)
Fluorochrome	Basic detection of HIV mRNA and protein – recommended for cell lines.	Minimum panel for CD4 T cell HIV translation-competent reservoir detection	Identification of memory phenotype of HIV^RNA+/Gag+ CD4
BUV395			CD3
BUV496			CD4
AF488	GagPol mRNA (Type 4)		CXCR5^*
PE	Gag Protein	Gag Protein	Gag Protein
PE-Cy7		CD4	ICOS
BV421			PD-1
BV510/eF506		Exclusion	Exclusion + Viability stain
BV605		CD3	CD27
BV711			CD45RA
AF647		GagPol mRNA (Type 1)	GagPol mRNA (Type 1)
eF780		Viability stain

Stained during stimulation.