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. 2017 Oct 12;6:e30688. doi: 10.7554/eLife.30688

Figure 3. Coupling between the R-domain and DBD in GR.

Figure 3.

(a) Schematic representation of competitive transfection assay design for constructs of the R-domains of isoforms A, B, C1, and C2 as well as the internally truncated R-domain linked to GR DBD or Gal4 DBD. (b) Experimental competitive transfection assay for constructs shown in a. The first 86 residues of R-domain present in GR A and B isoforms significantly increase the binding affinity of the DBD to GRE. Linking the R-domains to the Gal4 DBD ablate coupling. (c) Linkers of 11aa and 20aa were compared for the effect when connected alone to the GR DBD or used to link the R-domain (GR 1–97 segment) to the DBD. Linker length in panel a and all the other figures is 11aa. Error bars represent uncertainty of the individual fits.