Key resource table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (UCBPP-PA14 Pseudomonas aeruginosa) |
wild type (WT) | PMID: 7604262 | ||
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆phz | PMID: 16879411 | LD24 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1; ∆N1 | this study | LD1784 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN2; ∆N2 | this study | LD1614 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN3; ∆N3 | this study | LD1620 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN4; ∆N4 | this study | LD2833 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2; ∆N1∆N2 | this study | LD1888 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN4; ∆N1∆N4 | this study | LD1951 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN2∆ccoN4; ∆N2∆N4 | this study | LD1692 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN3∆ccoN4; ∆N3∆N4 | this study | LD1649 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆ccoN3;
∆N1∆N2∆N3 |
this study | LD1977 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆ccoN4;
∆N1∆N2∆N4 |
this study | LD1976 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆ccoN4
∆ccoN3; ∆N1∆N2∆N4∆N3 |
this study | LD2020 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆cco1cco2 | this study | LD1933 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆cox∆cyo∆cio | this study | LD2587 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆hcn | this study | LD2827 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆phz∆hcn | this study | LD2828 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN4∆hcn; ∆N4∆hcn | this study | LD2829 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆hcn;
∆N1∆N2∆hcn |
this study | LD2830 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆ccoN4
∆hcn; ∆N1∆N2∆N4∆hcn |
this study | LD2831 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆cco1cco2∆hcn | this study | LD2832 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
gacA::Tn | PMID: 16477005 | LD1560 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN4::ccoN4; ∆N4::N4 | this study | LD1867 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆ccoN4::
ccoN4; ∆N1∆N2∆N4::N4 |
this study | LD2576 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
MCS-gfp | this study | LD2820 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
Pcco1-gfp; cco1Pr-gfp | this study | LD2784 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
Pcco2-gfp; cco2Pr-gfp | this study | LD2786 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
PccoN4-gfp; ccoN4Pr-gfp | this study | LD2788 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
PA14-yfp | this study | LD2780 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2-yfp; ∆N1∆N2-yfp |
this study | LD2013 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN4-yfp; ∆N4-yfp | this study | LD2834 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆ccoN1∆ccoN2∆cco
N4-yfp; ∆N1∆N2∆N4-yfp |
this study | LD2136 | |
| strain, strain background (UCBPP-PA14 P. aeruginosa) |
∆cco1cco2-yfp | this study | LD2012 | |
| strain, strain background (Escherichia coli) |
UQ950 | other | From D. Lies, Caltech; LD44 | |
| strain, strain background (E. coli) |
BW29427 | other | From W. Metcalf, University of Illinois; LD661 | |
| strain, strain background (E. coli) |
β2155 | PMID: 8990308 | LD69 | |
| strain, strain background (E. coli) |
S17-1 | doi:10.1038/nbt1183-784 | LD2901 | |
| strain, strain background (Saccharomyces cerevisiae) |
InvSc1 | Invitrogen | LD676 | |
| recombinant DNA reagent | pMQ30 (plasmid) | PMID: 16820502 | For generation of deletion constructs listed above; further information can be found in the Materials and Methods section. |
|
| recombinant DNA reagent | pAKN69 (plasmid) | PMID: 15186351 | For generation of strains constitutively expression eyfp; further information can be found in the Materials and Methods section. |
|
| recombinant DNA reagent | pLD2722 (plasmid) | this study | For generation of gfp reporter constructs; further information can be found in the Materials and Methods section. |
|
| recombinant DNA reagent | pFLP2 (plasmid) | PMID: 9661666 | For generation of gfp reporter constructs; further information can be found in the Materials and Methods section. |
|
| software, algorithm | EggNOG Database | PMID: 26582926 | http://eggnogdb.embl.de/#/app/home | |
| software, algorithm | SensorTrace Profiling | Unisense | For data acquisition for redox and oxygen microprofiling; further information can be found in the Materials and Methods section. |
|
| other | Agar | Teknova | For colony morphology assays; further information can be found in the Materials and Methods section. |
|
| other | Lectin | Vector Laboratories | For visualization of matrix; further information can be found in the Materials and Methods section. |